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Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor
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Michael O. Daramola,
Chedly Tizaoui ,
Samuel A. Iwarere
Environmental Science and Pollution Research, Volume: 31, Issue: 37, Pages: 49811 - 49822
Swansea University Author:
Chedly Tizaoui
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DOI (Published version): 10.1007/s11356-024-34475-4
Abstract
Wastewater treatment plants (WWTPs) have been implicated as direct key reservoir of both antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) associated with human infection, as high concentrations of ARBs and ARGs have been detected in recycled hospital wastewater. Among the AR...
| Published in: | Environmental Science and Pollution Research |
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| ISSN: | 1614-7499 |
| Published: |
Springer Science and Business Media LLC
2024
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa69315 |
| Abstract: |
Wastewater treatment plants (WWTPs) have been implicated as direct key reservoir of both antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) associated with human infection, as high concentrations of ARBs and ARGs have been detected in recycled hospital wastewater. Among the ARBs, the carbapenem-resistant Acinetobacter baumannii has been ranked as priority 1 (critical) pathogen by the World Health Organization (WHO), due to its overwhelming burden on public health. Therefore, this study is aimed at investigating non-thermal plasma (NTP) technology as an alternative disinfection step to inactivate this bacterium and its ARGs. Culture-based method and PCR were employed in confirming the carbapenem resistance gene blaNDM-1 in A. baumannii (BAA 1605). Suspension of carbapenem-resistant A. baumannii (24 h culture) was prepared from the confirmed isolate and subjected to plasma treatment at varying time intervals (3 min, 6 min, 9 min, 12 min, and 15 min) in triplicates. The plasma-treated samples were evaluated for re-growth and the presence of the resistance gene. The treatment resulted in a 1.13 log reduction after 3 min and the highest log reduction of ≥ 8 after 15 min, and the results also showed that NTP was able to inactivate the blaNDM-1 gene. The log reduction and gel image results suggest that plasma disinfection has a great potential to be an efficient tertiary treatment step for WWTPs. |
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| Keywords: |
Acinetobacter baumannii; Carbapenem-resistant gene; Cold atmospheric plasma; Disinfection |
| College: |
Faculty of Science and Engineering |
| Funders: |
Open access funding provided by University of Pretoria. This research was supported by the Government of the United Kingdom through The Royal Society FLAIR award [FLR\R1\201683]. |
| Issue: |
37 |
| Start Page: |
49811 |
| End Page: |
49822 |